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SEM IMAGE ANALYSIS

 

Robbie discusses some of the knowledge that emerged from the SEM images whereas some findings remain inconclusive but pose further questions.

SEM findings - what we discovered
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SCALE AND UNITS

A basic SEM key

mag        - magnification, shown on the image (electron micrograph) as a scale bar, used to work out the actual                              magnification of the image since it scales with the size of the image

WD          - working distance between the sample and the lens

det ETD  - Everhart -Thornley Detector (high vacuum mode)

HV (kV)  - accelerating voltage for the electrons

HFW        - horizontal field width 

curr (pA) - beam current in picoampere

μm          - micrometre

Unit of measurement 

1/1000 m     = 1 mm (milimetre)

1/1000 mm = 1 μm (micrometre)

1/1000 μm  = 1 nm (nanometre)

SEED ACCESSION INFORMATION

 

Iris atropurpurea 

YS 10/11 - 001

Critically Endangered 

Israel

 

                               (without elaiosome) 

 

Orothamnus zeyheri 

RT 14 - 005

Vulnerable  

South Africa

 

Protea cryophila 

SHS 15 - 005

Near Threatened  

South Africa

 

Spatalla nubicola 

BMR 17 - 045

Near Threatened  

South Africa

 

Seed accession category used by Robbie-Blackhall-Miles. The letters signify the collector:

RT      Robbie Thomas

BMR  Robbie Blackhall-Miles and Ben Ram

SHS   Rod and Rachel Saunders of Silverhill Seeds

YS      Yuval Sapir of Tel Aviv Botanic Gardens

 

The number following is the year of collection i.e.10 is 2010. The third and final lot of numbers is the accession from that collector  i.e. RT14-002 is Robbie Thomas collected in 2014 and it was the second lot of seeds that came into collection from him from that year.

When Robbie sows the seeds they are given a individual batch number ie. BMR17-045.01 and when a seedling germinates it is given a individual code i.e. BMR17-045.01.01 which will stay with it for the rest of its life and allows him to track its progress.

  

 

Mimetes argenteus (with elaiosome) 

RT 14 - 002

Endangered 

South Africa

2018 © Raji Salan
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